Goat anti-Rat IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.56952-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.56952-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.56952-100mg | €1562.50 |
| Catalog No | CAB.56952 |
|---|---|
| Product Name | Goat anti-Rat IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Rat IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Rat |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300（PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Goat anti-Rat IgG antibody is designed for use in various applications, including ELISA and Western Blotting.
For ELISA, the recommended dilution is 1:5000-100000. Reagents and buffers required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the plate, followed by blocking and washing steps. The antibody is then incubated with the antigen, and CABter washing, the substrate is added for detection. Technical notes: it is essential to optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents and buffers required include the sample, transfer buffer, blocking buffer, and detection reagents. Sample preparation involves separating the proteins by SDS-PAGE, followed by transfer onto a membrane. The membrane is then blocked, and the antibody is incubated with the membrane. CABter washing, the detection reagent is added, and the signal is visualized. Technical notes: the transfer conditions and blocking buffer can significantly CABfect the outcome of the experiment, and optimization may be necessary.
Both applications require careful optimization of the antibody dilution and experimental conditions to achieve the best results.
Use assay conditions validated for ELISA WB, apply Goat anti-Rat IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-100000..
Precautions
Store at -20°C, handle with care, and use appropriate controls. Optimization of antibody dilution and experimental conditions is crucial for best results.
Validation Images