Donkey Anti-Rabbit IgG(Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.57755-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.57755-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.57755-5mL | €1125.00 |
| Catalog No | CAB.57755 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG(Immune serum) |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | Rabbit IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | Polyclonal Antibody |
| Reactivity | Rabbit |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)， is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Donkey Anti-Rabbit IgG (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-700000. The reagents required include the antigen of interest, coating buffer, blocking buffer, and substrate. The sample preparation involves coating the antigen onto the ELISA plate, followed by blocking and washing steps. The antibody incubation is typically done at room temperature for 1-2 hours, followed by washing and detection using a suitable substrate. Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, transfer buffer, blocking buffer, and detection reagents. The sample preparation involves separating the proteins using SDS-PAGE, followed by transferring the proteins onto a membrane. The antibody incubation is typically done at 4°C overnight, followed by washing and detection using a suitable detection reagent. Technical notes: Use a suitable positive control to ensure the specificity of the antibody.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antigen of interest, agarose, and a suitable buffer. The sample preparation involves preparing the antigen and antibody solutions, followed by loading them into the wells of the agarose plate. The antibody incubation is typically done at room temperature for 24-48 hours, followed by washing and visualization of the precipitin line. Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
Precautions
Store at 2-8°C, handle with care, use suitable controls, and optimize antibody dilution and incubation time to achieve the best results.
Validation Images