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CATEGORIES

Goat anti-Human C3 (Immune serum)

Product classification
Antibodies Secondary Antibody Immune Serum
Catalog No CAB.57971
Antibody overview
CAB.57971 Antibodies
Goat anti-Human C3 (Immune serum)
Ordering
Available Variants
1 Variant
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0.5mL
SKU
CAB.57971-0.5mL
Price
€362.50
Available – Dispatch in 14 days
Availability & Stock Pack SKU Price (EUR)
Available – Dispatch in 14 days
Global Network - International delivery
0.5mL CAB.57971-0.5mL €362.50
Quick page guide: Compare pack sizes, review validated applications and access product documents from this section.
Recommended Dilution
Double Immunodiffusion 1:16-32
Validated Application Key
Double Immunodiffusion
Validated application
Double Immunodiffusion - Validated application
Technical Specifications
Catalog No CAB.57971
Product Name Goat anti-Human C3 (Immune serum)
Isotype N/A
Calculated MW 187kDa
Immunogen Human C3
Public Immunogen Range N/A
Host Goat
Clone Type Polyclonal Antibody
Reactivity Human
Application Double Immunodiffusion
Subcellular Location N/A
Purification Method N/A
Storage Buffer N/A
Storage Shipped at 2-8℃,Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles).

Background

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. CABter activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.This product is reddish transparent liquid, containing 0.03% NaN3.

Protocol / Instructions

Recommended workflow
Optimized handling and experimental guidance
Review the suggested sequence below before starting the assay to keep sample handling, incubation, and downstream interpretation aligned.
Lab-ready steps

The Goat anti-Human C3 antibody is designed for use in various immunological applications. Below are the protocols for the validated application key provided.

Double Immunodiffusion Protocol

Reagents or buffers: Goat anti-Human C3 antibody, human C3 antigen, agar or agarose gel, electrophoresis buffer, and saline.

Sample preparation: Prepare the antigen and antibody samples according to the manufacturer's instructions.

Antibody incubation: Place the antibody and antigen samples in adjacent wells of the agar or agarose gel and incubate at room temperature for 24-48 hours.

Washing or detection: Observe the gel for precipitation lines, which indicate a positive reaction.

Technical notes: The optimal dilution of the antibody should be determined by the user, but a starting point of 1:16-32 is recommended.

Precautions

Store at 2-8°C. Handle with care, as the product contains 0.03% NaN3. Use appropriate controls and optimize the protocol as needed.

Validation Images

Goat anti-Human C3 (Immune serum) Figure 1
Fig.1. Validation data.