BSA/Solar Fluor647
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.58601-100ul | €87.50 |
| Catalog No | CAB.58601 |
|---|---|
| Product Name | BSA/Solar Fluor647 |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | N/A |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃， Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction to BSA/Solar Fluor647 Antibody
The BSA/Solar Fluor647 antibody is designed for the detection of Bovine Serum Albumin (BSA) and is validated for use in Immunofluorescence (IF) applications.
- Reagents or Buffers: Phosphate-buffered saline (PBS), Triton X-100 for permeabilization if necessary, and a mounting medium with DAPI for nuclear staining.
- Sample Preparation: Fix cells or tissue sections with a suitable fixative such as paraformaldehyde, followed by permeabilization if required.
- Antibody Incubation: Incubate the sample with the BSA/Solar Fluor647 antibody at a dilution of 1:50-500 in PBS containing 1% BSA for 1 hour at room temperature or overnight at 4°C.
- Washing or Detection: Wash the samples thoroughly with PBS, and then proceed with fluorescence microscopy. The Solar Fluor647 fluorophore has an excitation maximum at approximately 652 nm and an emission maximum at approximately 672 nm.
- Technical Notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions and the expression level of BSA in the sample.
## Troubleshooting and Optimization
For optimal results, ensure that the sample is properly fixed and permeabilized. The choice of fixative and the fixation time can significantly CABfect the staining outcome. Additionally, the use of a blocking step with BSA or normal serum can help reduce background staining.
Use assay conditions validated for IF, apply BSA/Solar Fluor647 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for specific applications to minimize background and ensure specific staining.
Validation Images