Donkey Anti-Guinea Pig IgG/Gold
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.58693-500ul | €150.00 |
| Catalog No | CAB.58693 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/Gold |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | ICA;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/Gold antibody is designed for use in various immunocytochemical applications.
The recommended dilution for this antibody is 1:20-200 for both Immunocytochemistry (ICA) and Immunoelectron Microscopy (IEM).
Reagents or buffers: phosphate-buffered saline (PBS), primary antibody, secondary antibody (Donkey Anti-Guinea Pig IgG/Gold), and mounting medium.
Sample preparation: fix cells or tissue sections with a suitable fixative, permeabilize with a detergent if necessary, and block non-specific binding sites.
Antibody incubation: incubate samples with the primary antibody, followed by the Donkey Anti-Guinea Pig IgG/Gold secondary antibody.
Washing or detection: wash samples thoroughly between incubations, and detect the signal using a suitable method.
Technical notes: optimize the primary and secondary antibody dilutions for the best signal-to-noise ratio.
Reagents or buffers: PBS, primary antibody, secondary antibody (Donkey Anti-Guinea Pig IgG/Gold), and a suitable electron microscopy embedding medium.
Sample preparation: fix cells or tissue sections with a suitable fixative, and block non-specific binding sites.
Antibody incubation: incubate samples with the primary antibody, followed by the Donkey Anti-Guinea Pig IgG/Gold secondary antibody.
Washing or detection: wash samples thoroughly between incubations, and detect the signal using electron microscopy.
Technical notes: optimize the primary and secondary antibody dilutions for the best signal-to-noise ratio, and consider using a post-embedding staining method for better preservation of ultrastructure.
Use assay conditions validated for ICA, apply Donkey Anti-Guinea Pig IgG/Gold at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICA=1:20-200;
IEM=1:20-200.
Use assay conditions validated for IEM, apply Donkey Anti-Guinea Pig IgG/Gold at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: ICA=1:20-200;
IEM=1:20-200.
Precautions
Store at 2-8°C, handle with care, and use appropriate controls. Optimize antibody dilutions and incubation conditions for best results.
Validation Images