Donkey Anti-Guinea Pig IgG/Cy5.5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.59348-100ul | €200.00 |
| Catalog No | CAB.59348 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/Cy5.5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/Cy5.5 is a highly specific antibody conjugate designed for various applications.
For IF, the following steps are recommended:
- Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, and a suitable mounting medium.
- Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, followed by permeabilization if necessary.
- Antibody incubation: Incubate the sample with the Donkey Anti-Guinea Pig IgG/Cy5.5 at a dilution of 1:100-1000 in PBS for 1 hour at room temperature or overnight at 4°C.
- Washing or detection: Wash the sample thoroughly with PBS, and proceed with imaging using a fluorescence microscope.
- Technical notes: Optimize the dilution of the antibody and the incubation time based on the specific application and the intensity of the signal.
## Technical Considerations
Given the single validated application, ensure that the protocol is optimized for the specific experimental conditions. Consider the use of controls to validate the specificity of the staining.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/Cy5.5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for specific applications to avoid non-specific binding.
Validation Images