Goat anti-Pig IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.3ml | CAB.59572-0.3ml | €47.50 |
| Catalog No | CAB.59572 |
|---|---|
| Product Name | Goat anti-Pig IgG/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Pig IgG/RBITC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
Reagents or buffers: Goat anti-Pig IgG/RBITC antibody, phosphate-buffered saline (PBS), mounting medium.
Sample preparation: Fix cells or tissue sections with a suitable fixative, permeabilize if necessary, and block non-specific binding sites.
Antibody incubation: Incubate samples with the Goat anti-Pig IgG/RBITC antibody at a dilution of 1:50-500 in PBS for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples thoroughly with PBS, and mount with a suitable mounting medium. Visualize using a fluorescence microscope.
Reagents or buffers: Goat anti-Pig IgG/RBITC antibody, phosphate-buffered saline (PBS), flow cytometry buffer.
Sample preparation: Prepare cell suspensions and block non-specific binding sites if necessary.
Antibody incubation: Incubate cells with the Goat anti-Pig IgG/RBITC antibody at a dilution of 1:50-500 in flow cytometry buffer for 15-30 minutes at 4°C.
Washing or detection: Wash cells thoroughly with flow cytometry buffer, and analyze using a flow cytometer.
Technical notes: Optimize antibody dilutions and incubation times for specific applications and samples. Use appropriate controls to verify specificity and sensitivity.
Use assay conditions validated for IF, apply Goat anti-Pig IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Use assay conditions validated for FCM, apply Goat anti-Pig IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, optimize antibody dilutions, and use appropriate controls to ensure specificity and sensitivity.
Validation Images