Donkey Anti-Goat IgG/PE-Cy5.5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.59987-100ul | €267.50 |
| Catalog No | CAB.59987 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/PE-Cy5.5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/PE-Cy5.5 is a highly specific antibody conjugate designed for various applications. Here, we provide protocols for its validated applications.
For IF, the following steps are recommended:
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde for 15 minutes, followed by permeabilization with 0.1% Triton X-100 for 10 minutes.
Antibody incubation: Incubate samples with the Donkey Anti-Goat IgG/PE-Cy5.5 at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: Wash samples three times with PBS, then mount with mounting medium and analyze using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions and sample type.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/PE-Cy5.5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for each application to minimize background and ensure specificity.
Validation Images