Goat anti-Equine IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.3ml | CAB.60055-0.3ml | €55.00 |
| Catalog No | CAB.60055 |
|---|---|
| Product Name | Goat anti-Equine IgG/RBITC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Equine IgG/RBITC antibody is designed for use in various applications. Below are the protocols for the validated application.
For IF, the following steps should be taken:
Reagents or buffers: Phosphate-buffered saline (PBS), mounting medium, and a fluorescence microscope.
Sample preparation: Fix cells or tissue sections with a suitable fixative, then permeabilize if necessary.
Antibody incubation: Dilute the Goat anti-Equine IgG/RBITC antibody to 1:50-500 in PBS and incubate for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples thoroughly with PBS, then mount and visualize using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific experimental conditions.
Use assay conditions validated for IF, apply Goat anti-Equine IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution for specific applications.
Validation Images