BSA/Solar Fluor488
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.60956-100ul | €87.50 |
| Catalog No | CAB.60956 |
|---|---|
| Product Name | BSA/Solar Fluor488 |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | N/A |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃， Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction
The BSA/Solar Fluor488 antibody is a valuable tool for detecting Bovine Serum Albumin (BSA) in various applications. This protocol outlines the steps for using this antibody in Immunofluorescence (IF) applications.
For IF applications, the BSA/Solar Fluor488 antibody can be used to detect BSA in fixed cells or tissues. The recommended dilution for IF is 1:50-500.
Reagents or buffers needed include the BSA/Solar Fluor488 antibody, a suitable fixation method (e.g., paraformaldehyde), a permeabilization agent (e.g., Triton X-100), and a mounting medium.
Sample preparation involves fixing and permeabilizing the cells or tissues, followed by blocking with a suitable blocking buffer.
Antibody incubation is typically performed at 4°C overnight or at room temperature for 1-2 hours.
Washing is done with a suitable washing buffer, and detection can be achieved using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for the specific application. Use a suitable negative control to verify the specificity of the staining.
Use assay conditions validated for IF, apply BSA/Solar Fluor488 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, optimize antibody dilution and incubation time, and use suitable controls to ensure specificity.
Validation Images