Anti-CAV1 Recombinant Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.60995-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.60995-100ul | €437.50 |
| Catalog No | CAB.60995 |
|---|---|
| Product Name | Anti-CAV1 Recombinant Antibody |
| Isotype | IgG |
| Calculated MW | N/A |
| Immunogen | A synthetic peptide of human CAV1 |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Recombinant Antibody |
| Reactivity | Human |
| Application | WB;IHC-P;IF/ICC |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN，an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.
Protocol / Instructions
Reagents or buffers: RIPA buffer, protease inhibitor cocktail, SDS-PAGE gel, transfer buffer, blocking buffer, and ECL substrate.
Sample preparation: Lyse cells in RIPA buffer with protease inhibitors, then separate proteins by SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with Anti-CAV1 Recombinant Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash the membrane with TBST, then incubate with a secondary antibody conjugated to HRP, and detect using ECL substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, blocking buffer, and DAB substrate.
Sample preparation: Fix and embed tissue samples, then section and deparaffinize with xylene and ethanol.
Antibody incubation: Incubate sections with Anti-CAV1 Recombinant Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash sections with PBS, then incubate with a secondary antibody conjugated to HRP, and detect using DAB substrate.
Technical notes: Perform antigen retrieval using heat-mediated or enzymatic methods to enhance staining.
Reagents or buffers: PBS, Triton X-100, blocking buffer, and fluorescently labeled secondary antibody.
Sample preparation: Fix and permeabilize cells with PBS and Triton X-100, then block with blocking buffer.
Antibody incubation: Incubate cells with Anti-CAV1 Recombinant Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash cells with PBS, then incubate with a fluorescently labeled secondary antibody, and detect using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for best results, and consider using mounting media with DAPI to counterstain nuclei.
Precautions
Store at -20°C, handle with gloves, use proper controls, and optimize antibody dilution and incubation time for each application to ensure best results.
Validation Images