Anti-AAAS Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.61469-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.61469-100ul | €437.50 |
| Catalog No | CAB.61469 |
|---|---|
| Product Name | Anti-AAAS Monoclonal Antibody |
| Isotype | IgG2a |
| Calculated MW | N/A |
| Immunogen | Recombinant protein of human AAAS |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human |
| Application | WB;IF/ICC;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
Part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression，sister chromatid cohesion，and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase，facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed:38554706).
Protocol / Instructions
The Anti-AAAS Monoclonal Antibody is a mouse-derived antibody that targets the AAAS protein in human samples. This antibody has been validated for use in Western Blotting (WB), Immunofluorescence/Immunocytochemistry (IF/ICC), and Flow Cytometry (FCM) applications.
For Western Blotting, prepare samples by lysing cells in a suitable buffer such as RIPA buffer. Separate proteins by SDS-PAGE and transfer to a PVDF membrane. Block the membrane with 5% non-fat milk in TBS-T for 1 hour. Incubate the membrane with the Anti-AAAS Monoclonal Antibody at a suitable dilution in TBS-T for 2 hours at room temperature. Wash the membrane three times with TBS-T and detect using a suitable secondary antibody conjugated to HRP. Develop the blot using a chemiluminescent substrate.
For Immunofluorescence/Immunocytochemistry, fix cells with 4% paraformaldehyde for 15 minutes and permeabilize with 0.1% Triton X-100 for 10 minutes. Block cells with 5% non-fat milk in PBS for 1 hour. Incubate cells with the Anti-AAAS Monoclonal Antibody at a suitable dilution in PBS for 2 hours at room temperature. Wash cells three times with PBS and detect using a suitable secondary antibody conjugated to a fluorescent dye. Mount cells with a suitable mounting medium and visualize using a fluorescence microscope.
For Flow Cytometry, prepare cells by washing with PBS and fixing with 4% paraformaldehyde for 15 minutes. Permeabilize cells with 0.1% Triton X-100 for 10 minutes. Block cells with 5% non-fat milk in PBS for 1 hour. Incubate cells with the Anti-AAAS Monoclonal Antibody at a suitable dilution in PBS for 2 hours at room temperature. Wash cells three times with PBS and detect using a suitable secondary antibody conjugated to a fluorescent dye. Analyze cells using a flow cytometer.
Technical notes: The optimal dilution of the Anti-AAAS Monoclonal Antibody should be determined by the user for each specific application. It is recommended to use a positive control sample to verify the specificity of the antibody.
Precautions
Store at 2-8°C. Handle with care to avoid contamination. Use appropriate controls and optimize antibody dilution for each application.
Validation Images