Donkey Anti-Rabbit IgG/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.62710-50ul | €272.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.62710-100ul | €27.50 |
| Catalog No | CAB.62710 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG/HRP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | ELISA;IHC-F;IHC-P;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Rabbit IgG/HRP is a versatile antibody conjugate that can be used in various applications, including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry-Paraffin (IHC-P), and Immunohistochemistry-Frozen (IHC-F) sections.
For Western Blotting, the recommended dilution is 1:1000-10000. Reagents needed include the antibody, blocking buffer, and substrate for HRP. Sample preparation involves separating proteins by SDS-PAGE and transferring them to a membrane. The membrane is then blocked, incubated with the Donkey Anti-Rabbit IgG/HRP at the recommended dilution, and washed. Detection is achieved using an HRP substrate. Technical notes: Optimize the blocking conditions and antibody dilution to minimize background and enhance specific signal.
For ELISA, the recommended dilution is 1:1000-10000. Reagents needed include the antibody, coating buffer, blocking buffer, and substrate for HRP. Sample preparation involves coating the plate with antigen, blocking, and then adding the primary rabbit antibody followed by the Donkey Anti-Rabbit IgG/HRP. Washing and detection steps follow. Technical notes: Ensure proper plate washing between steps to avoid non-specific binding.
For IHC-P, the recommended dilution is 1:100-1000. Reagents needed include the antibody, antigen retrieval buffer, and a substrate for HRP. Sample preparation involves deparaffinization, antigen retrieval, and blocking. The Donkey Anti-Rabbit IgG/HRP is then applied, followed by washing and detection. Technical notes: Antigen retrieval methods and times may need optimization based on the specific tissue and antigen.
For IHC-F, the recommended dilution is 1:100-1000. Reagents needed include the antibody, blocking buffer, and a substrate for HRP. Sample preparation involves fixing and permeabilizing the tissue sections, followed by blocking and incubation with the primary rabbit antibody and then the Donkey Anti-Rabbit IgG/HRP. Washing and detection steps follow. Technical notes: Fixation and permeabilization conditions can significantly CABfect the outcome and may require optimization.
Precautions
Store at 2-8°C. Handle with care to avoid contamination. Use appropriate controls and optimize antibody dilutions for specific applications to ensure reliable results.
Validation Images