BSA/Solar Fluor647
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.62854-100ul | €87.50 |
| Catalog No | CAB.62854 |
|---|---|
| Product Name | BSA/Solar Fluor647 |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | N/A |
| Clone Type | N/A |
| Reactivity | N/A |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃， Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction
The BSA/Solar Fluor647 antibody is a valuable tool for detecting Bovine Serum Albumin (BSA) in various applications. This protocol outlines the steps for using this antibody in Immunofluorescence (IF) applications.
For IF applications, the BSA/Solar Fluor647 antibody can be used to detect BSA in fixed cells or tissues. The recommended dilution for IF is 1:50-500.
- Reagents or Buffers: Phosphate-buffered saline (PBS), Triton X-100, mounting medium.
- Sample Preparation: Fix cells or tissues with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100 in PBS.
- Antibody Incubation: Incubate the sample with the BSA/Solar Fluor647 antibody at the recommended dilution for 1 hour at room temperature or overnight at 4°C.
- Washing or Detection: Wash the sample with PBS, and detect the signal using a fluorescence microscope.
- Technical Notes: Optimize the antibody dilution and incubation time for specific applications. Use appropriate controls to verify the specificity of the staining.
## Troubleshooting
If the signal is weak, try increasing the antibody concentration or extending the incubation time. If background fluorescence is high, try reducing the antibody concentration or using a different mounting medium.
Use assay conditions validated for IF, apply BSA/Solar Fluor647 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for specific applications.
Validation Images