Goat Anti-Mouse IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.63174-100ul | €200.00 |
| Catalog No | CAB.63174 |
|---|---|
| Product Name | Goat Anti-Mouse IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgG |
| Application | FCM;IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgG/APC is a versatile antibody that can be used in various applications, including Flow Cytometry (FCM) and Immunofluorescence (IF).
For FCM, the recommended dilution is 1:100-1000. Reagents needed include the antibody, phosphate-buffered saline (PBS), and a flow cytometer. Sample preparation involves washing cells with PBS and resuspending them in a suitable buffer. Antibody incubation is typically performed at 4°C for 30 minutes. Washing is done with PBS, and detection is carried out using a flow cytometer. Technical notes: it is essential to optimize the antibody dilution and incubation time for specific cell types and experimental conditions.
For IF, the recommended dilution is also 1:100-1000. Reagents needed include the antibody, PBS, a fluorescent microscope, and mounting medium. Sample preparation involves fixing cells or tissue sections with a suitable fixative, followed by permeabilization and blocking. Antibody incubation is typically performed at room temperature for 1-2 hours or at 4°C overnight. Washing is done with PBS, and detection is carried out using a fluorescent microscope. Technical notes: it is crucial to optimize the antibody dilution, incubation time, and washing conditions to minimize background fluorescence and enhance specific staining.
Use assay conditions validated for FCM, apply Goat Anti-Mouse IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for IF, apply Goat Anti-Mouse IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions to minimize non-specific binding and ensure optimal results.
Validation Images