Avidin/FITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.67847-0.1ml | €75.00 |
| Catalog No | CAB.67847 |
|---|---|
| Product Name | Avidin/FITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Avidin |
| Clone Type | N/A |
| Reactivity | Avidin |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
## Introduction
The Avidin/FITC antibody is a valuable tool for various applications, including Immunofluorescence (IF). This protocol outlines the steps for using this antibody in IF.
For IF, the following steps can be followed:
- Reagents or buffers: PBS, Triton X-100 for permeabilization, and a mounting medium with DAPI for nuclear staining.
- Sample preparation: Fix cells with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block with 5% BSA.
- Antibody incubation: Incubate the Avidin/FITC antibody at a dilution of 1:100-1000 in PBS with 1% BSA for 1 hour at room temperature.
- Washing or detection: Wash cells three times with PBS, then detect using a fluorescence microscope.
- Technical notes: Optimize the antibody dilution and incubation time for specific cell types and experimental conditions.
## Notes on Other Applications
Although the provided validated application key is IF, the Avidin/FITC antibody can also be used in other applications such as Western Blotting (WB), ELISA, and Immunohistochemistry (IHC). For these applications, refer to the recommended dilutions and established protocols.
Use assay conditions validated for IF, apply Avidin/FITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB=1:1000-10000;
ELISA=1:1000-10000;
IHC-P=1:100-1000;
IHC-F=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation times for best results.
Validation Images