Goat Anti-Mouse IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.68067-100ul | €87.50 |
| Catalog No | CAB.68067 |
|---|---|
| Product Name | Goat Anti-Mouse IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Mouse IgG |
| Application | IHC-F;IHC-P;WB;ELISA |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Mouse IgG/AP is a versatile antibody that can be used in various applications.
For Western blotting, use a dilution of 1:1000 to 1:10000. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE. Transfer the proteins to a membrane and block with a blocking buffer. Incubate the membrane with the Goat Anti-Mouse IgG/AP antibody, followed by washing and detection using a suitable substrate.
For Immunohistochemistry on paraffin-embedded tissues (IHC-P), use a dilution of 1:100 to 1:1000. Deparaffinize and rehydrate tissue sections, then perform antigen retrieval if necessary. Block the sections with a blocking buffer and incubate with the Goat Anti-Mouse IgG/AP antibody. CABter washing, detect the signal using a suitable chromogen.
For Immunohistochemistry on frozen tissues (IHC-F), use a dilution of 1:100 to 1:1000. Fix and permeabilize tissue sections, then block with a blocking buffer. Incubate the sections with the Goat Anti-Mouse IgG/AP antibody, followed by washing and detection using a fluorescent secondary antibody.
For ELISA, use a dilution of 1:1000 to 1:10000. Coat the ELISA plate with the antigen, then block with a blocking buffer. Incubate the plate with the Goat Anti-Mouse IgG/AP antibody, followed by washing and detection using a suitable substrate.
Technical notes: Optimize the dilution and incubation time for each application to achieve the best results.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time to minimize background and ensure specific binding.
Validation Images