Donkey Anti-Guinea Pig IgG/PE-Cy5.5
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.68167-100ul | €267.50 |
| Catalog No | CAB.68167 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/PE-Cy5.5 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/PE-Cy5.5 antibody is a valuable tool for various immunofluorescence applications.
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, PE-Cy5.5-conjugated secondary antibody.
Sample preparation: fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100.
Antibody incubation: incubate samples with primary antibody followed by Donkey Anti-Guinea Pig IgG/PE-Cy5.5 at a dilution of 1:100-1000.
Washing or detection: wash samples with PBS, detect using a fluorescence microscope.
Technical notes: optimize antibody dilution and incubation time for specific applications.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/PE-Cy5.5 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, optimize antibody dilution and incubation time.
Validation Images