Anti-AARS2 Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.69008-50ul | €417.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.69008-100ul | €700.00 |
| Catalog No | CAB.69008 |
|---|---|
| Product Name | Anti-AARS2 Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 107kDa |
| Immunogen | Recombinant protein of human AARS2 |
| Public Immunogen Range | 841-985aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB |
| Subcellular Location | Mitochondrion |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases play critical roles in mRNA translation by charging tRNAs with their cognate amino acids. The encoded protein is a mitochondrial enzyme that specifically aminoacylates alanyl-tRNA. Mutations in this gene are a cause of combined oxidative phosphorylation deficiency 8.
Protocol / Instructions
The Anti-AARS2 Polyclonal Antibody is a rabbit-derived antibody raised against recombinant protein of human AARS2. It has been validated for use in ELISA and Western Blotting (WB) applications.
For ELISA, the recommended dilution is 1:500-2000. Reagents and buffers required include the Anti-AARS2 Polyclonal Antibody, antigen (recombinant human AARS2 protein or cell lysates), ELISA plates, blocking buffer, washing buffer, and substrate. Sample preparation involves coating the ELISA plates with the antigen overnight at 4°C. Antibody incubation is performed by adding the diluted Anti-AARS2 Polyclonal Antibody to the wells and incubating for 1 hour at room temperature. Washing is done with washing buffer, and detection is achieved using a suitable substrate. Technical notes: Optimize the coating antigen concentration and antibody dilution to achieve the best signal-to-noise ratio.
For Western Blotting, the recommended dilution is 1:500-2000. Reagents and buffers required include the Anti-AARS2 Polyclonal Antibody, sample lysates, SDS-PAGE gel, transfer buffer, blocking buffer, and washing buffer. Sample preparation involves resolving the samples on an SDS-PAGE gel and transferring them to a membrane. Antibody incubation is performed by adding the diluted Anti-AARS2 Polyclonal Antibody to the membrane and incubating overnight at 4°C. Washing is done with washing buffer, and detection is achieved using a suitable secondary antibody and substrate. Technical notes: Use a suitable positive control to confirm the specificity of the antibody, and optimize the blocking conditions to reduce non-specific binding.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution for best results.
Validation Images