ActivAbTMGoat Anti BSA/Solar Fluor 568
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20T | CAB.69565-20T | €335.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.69565-100T | €750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.69565-100ul | €87.50 |
| Catalog No | CAB.69565 |
|---|---|
| Product Name | ActivAbTMGoat Anti BSA/Solar Fluor 568 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | BSA |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The ActivAbTMGoat Anti BSA/Solar Fluor 568 is a highly specific antibody conjugate designed for various applications. Below are the protocols for the validated application.
For IF, the following steps are recommended:
- Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde (PFA) for fixation, 0.1% Triton X-100 for permeabilization, and a blocking buffer (e.g., 5% normal serum or BSA in PBS).
- Sample preparation: Fix cells or tissue sections with 4% PFA for 10-15 minutes, followed by permeabilization with 0.1% Triton X-100 for 10 minutes. Block with the blocking buffer for 30 minutes to 1 hour.
- Antibody incubation: Dilute the ActivAbTMGoat Anti BSA/Solar Fluor 568 in the blocking buffer at a concentration of 1:50-500, as recommended. Incubate the samples with the diluted antibody for 1-2 hours at room temperature or overnight at 4°C.
- Washing or detection: Wash the samples thoroughly with PBS CABter the primary antibody incubation. Since the antibody is conjugated with Solar Fluor 568, no secondary antibody is needed. Proceed with nuclear staining (e.g., DAPI) if required, followed by mounting and imaging.
## Technical Notes
- The optimal dilution of the antibody may vary depending on the specific application and the experimental setup. It is recommended to perform a titration to find the best dilution for the particular use.
- Controls, such as omitting the primary antibody or using an isotype control, should be included to verify the specificity of the staining.
Use assay conditions validated for IF, apply ActivAbTMGoat Anti BSA/Solar Fluor 568 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 4°C, handle with care, and optimize antibody dilution for specific applications to avoid non-specific binding.
Validation Images