Goat Anti-Rabbit IgG/RBITC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.3ml | CAB.70110-0.3ml | €42.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
3ml | CAB.70110-3ml | €312.50 |
| Catalog No | CAB.70110 |
|---|---|
| Product Name | Goat Anti-Rabbit IgG/RBITC |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Rabbit IgG/RBITC antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block non-specific binding sites.
Antibody incubation: Incubate samples with the Goat Anti-Rabbit IgG/RBITC antibody at a dilution of 1:50-500 in PBS for 1-2 hours at room temperature or overnight at 4°C.
Washing or detection: Wash samples thoroughly with PBS, and detect the signal using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers: Phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), and 0.1% sodium azide.
Sample preparation: Prepare cell suspensions and block non-specific binding sites with 1% BSA.
Antibody incubation: Incubate cells with the Goat Anti-Rabbit IgG/RBITC antibody at a dilution of 1:50-500 in PBS for 30 minutes to 1 hour on ice.
Washing or detection: Wash cells thoroughly with PBS, and detect the signal using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for specific cell types and experimental conditions.
Use assay conditions validated for IF, apply Goat Anti-Rabbit IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Use assay conditions validated for FCM, apply Goat Anti-Rabbit IgG/RBITC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:50-500;
IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific applications to avoid non-specific binding.
Validation Images