Goat Anti-Human IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.73245-100ul | €87.50 |
| Catalog No | CAB.73245 |
|---|---|
| Product Name | Goat Anti-Human IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgG/AP antibody is designed for use in various applications, including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry on Paraffin sections (IHC-P), and Immunohistochemistry on Frozen sections (IHC-F). The recommended dilution for each application is provided, but optimization may be required for specific experimental conditions.
For Western Blotting, dilute the antibody to 1:1000-10000 in the recommended buffer. Sample preparation involves lysing cells or tissues in an appropriate lysis buffer, followed by separation of proteins by SDS-PAGE and transfer to a membrane. Incubate the membrane with the diluted antibody for 1-2 hours at room temperature or overnight at 4°C. CABter washing, detect the signal using an alkaline phosphatase substrate.
In ELISA, the antibody is used at a dilution of 1:1000-10000. Prepare the antigen by coating the plate with the human IgG protein. Block non-specific binding sites and then incubate with the diluted antibody. CABter washing, add an alkaline phosphatase substrate to detect the bound antibody.
For Immunohistochemistry on Paraffin sections, the antibody is used at 1:100-1000 dilution. Deparaffinize and rehydrate the tissue sections, followed by antigen retrieval if necessary. Block endogenous peroxidase activity and non-specific binding sites. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. CABter washing, detect the signal using an alkaline phosphatase substrate.
In Immunohistochemistry on Frozen sections, the antibody is used at a dilution of 1:100-1000. Fix the frozen sections in an appropriate fixative, followed by blocking of non-specific binding sites. Incubate the sections with the diluted antibody for 30 minutes to 1 hour at room temperature. CABter washing, detect the signal using an alkaline phosphatase substrate.
Technical notes: Optimization of the antibody dilution and incubation time may be necessary for specific applications. Controls, including positive and negative controls, should be included to validate the results.
Precautions
Store at 2-8°C. Handle with care, avoiding freeze-thaw cycles. Include proper controls and optimize conditions for each application.
Validation Images