Goat Anti-Rabbit IgG/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.73514-100ul | €87.50 |
| Catalog No | CAB.73514 |
|---|---|
| Product Name | Goat Anti-Rabbit IgG/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Rabbit IgG |
| Application | IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Rabbit IgG/AP is a versatile antibody that can be used in various applications, including Western Blotting (WB), Enzyme-Linked Immunosorbent Assay (ELISA), Immunohistochemistry-Paraffin (IHC-P), and Immunohistochemistry-Frozen (IHC-F) sections.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and alkaline phosphatase substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a membrane.
Antibody incubation: Incubate the membrane with the Goat Anti-Rabbit IgG/AP at a dilution of 1:1000-10000 in TBST with 5% skim milk for 1 hour at room temperature.
Washing or detection: Wash the membrane three times with TBST and detect using alkaline phosphatase substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Reagents or buffers: Coating buffer, blocking buffer, and alkaline phosphatase substrate.
Sample preparation: Coat the plate with antigen and block with blocking buffer.
Antibody incubation: Incubate the plate with the Goat Anti-Rabbit IgG/AP at a dilution of 1:1000-10000 in blocking buffer for 1 hour at room temperature.
Washing or detection: Wash the plate three times with washing buffer and detect using alkaline phosphatase substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Reagents or buffers: Xylene, ethanol, and alkaline phosphatase substrate.
Sample preparation: Deparaffinize and rehydrate tissue sections.
Antibody incubation: Incubate the sections with the Goat Anti-Rabbit IgG/AP at a dilution of 1:100-1000 in antibody diluent for 30 minutes at room temperature.
Washing or detection: Wash the sections three times with phosphate-buffered saline (PBS) and detect using alkaline phosphatase substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Reagents or buffers: Acetone, and alkaline phosphatase substrate.
Sample preparation: Fix tissue sections with acetone.
Antibody incubation: Incubate the sections with the Goat Anti-Rabbit IgG/AP at a dilution of 1:100-1000 in antibody diluent for 30 minutes at room temperature.
Washing or detection: Wash the sections three times with phosphate-buffered saline (PBS) and detect using alkaline phosphatase substrate.
Technical notes: Optimize the dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images