Goat anti-Human IgM (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.73702-0.5mL | €212.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.73702-1mL | €1500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.73702-5mL | €1500.00 |
| Catalog No | CAB.73702 |
|---|---|
| Product Name | Goat anti-Human IgM (Immune serum) |
| Isotype | N/A |
| Calculated MW | 950kDa |
| Immunogen | Human IgM purified from human plasma |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it‘s large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it‘s high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Human IgM (Immune serum) is a versatile antibody that can be used in various applications such as ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-50000. The reagents required include the antibody, antigen, and substrate. The sample preparation involves coating the antigen onto the ELISA plate, followed by blocking with a suitable blocking buffer. The antibody incubation is done at a dilution of 1:5000-50000 for 1-2 hours at room temperature. Washing is done with a suitable washing buffer, and detection is done using a substrate. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the antibody, sample, and membrane. The sample preparation involves separating the proteins using SDS-PAGE, followed by transferring the proteins onto a membrane. The antibody incubation is done at a dilution of 1:1000-20000 for 1-2 hours at room temperature. Washing is done with a suitable washing buffer, and detection is done using a suitable detection method. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antibody, antigen, and agar. The sample preparation involves preparing the antigen and antibody solutions, followed by adding them to the agar gel. The antibody incubation is done at a dilution of 1:4-32 for 24-48 hours at room temperature. Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images