Donkey Anti-Goat IgG/PE-Cy3
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.74440-100ul | €267.50 |
| Catalog No | CAB.74440 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/PE-Cy3 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/PE-Cy3 antibody is a versatile reagent for various applications. For optimal results, follow the protocols outlined below for each validated application.
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block non-specific binding sites.
Antibody incubation: incubate samples with Donkey Anti-Goat IgG/PE-Cy3 at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: wash samples with PBS and mount with mounting medium.
Technical notes: optimize antibody dilution and incubation time for specific applications.
Reagents or buffers: phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), and 0.1% sodium azide.
Sample preparation: prepare cell suspensions and block non-specific binding sites with 1% BSA.
Antibody incubation: incubate cells with Donkey Anti-Goat IgG/PE-Cy3 at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: wash cells with PBS and analyze by flow cytometry.
Technical notes: optimize antibody dilution and incubation time for specific cell types and applications.
For both IF and FCM applications, it is essential to handle the antibody with care and follow proper protocols to ensure optimal results.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/PE-Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/PE-Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific applications to avoid non-specific binding and ensure optimal results.
Validation Images