Rabbit anti-Avidin (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.75301-0.5mL | €412.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.75301-1mL | €2750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.75301-5mL | €2750.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.75301-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.75301-100ul | €335.00 |
| Catalog No | CAB.75301 |
|---|---|
| Product Name | Rabbit anti-Avidin (Immune serum) |
| Isotype | N/A |
| Calculated MW | 60kDa |
| Immunogen | Avidin |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Avidin |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds， reptiles and amphibians deposited in the whites of their eggs. In chicken egg white， avidin makes up approximately 0.05% of total protein (approximately 1.8 mg per egg). The tetrameric protein contains four identical subunits (homotetramer)， each of which can bind to biotin (Vitamin B7， vitamin H) with a high degree of CABfinity and specificity. In its tetrameric form， avidin is estimated to be between 66–69 kDa in size.Ten percent of the molecular weight is attributed to carbohydrate content composed of four to five mannose and three N-acetylglucosamine residues. The carbohydrate moieties of avidin contain at least three unique oligosaccharide structural types that are similar in structure and composition.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Rabbit anti-Avidin (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-700000. The reagents required include the antigen, secondary antibody conjugated to an enzyme, substrate, and stop solution. Sample preparation involves coating the antigen onto the ELISA plate, followed by blocking and washing steps. The primary antibody is then added, and CABter incubation, the plate is washed, and the secondary antibody is added. The enzyme substrate is then added, and the reaction is stopped. The absorbance is measured, and the results are calculated.
Reagents or buffers required include coating buffer, blocking buffer, washing buffer, and substrate. Technical notes: it is essential to optimize the coating antigen concentration, primary and secondary antibody dilutions, and incubation times to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, primary antibody, secondary antibody conjugated to an enzyme, substrate, and membrane. Sample preparation involves separating the proteins using SDS-PAGE, followed by transferring the proteins onto a membrane. The membrane is then blocked, and the primary antibody is added. CABter incubation, the membrane is washed, and the secondary antibody is added. The enzyme substrate is then added, and the results are visualized.
Reagents or buffers required include transfer buffer, blocking buffer, washing buffer, and substrate. Technical notes: it is crucial to optimize the sample preparation, primary and secondary antibody dilutions, and incubation times to achieve the best results.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antigen, primary antibody, and agar. Sample preparation involves preparing the antigen and antibody solutions. The solutions are then added to the agar plate, and the plate is incubated. The results are visualized as a precipitin line.
Reagents or buffers required include agar, antigen, and primary antibody. Technical notes: it is essential to optimize the antigen and antibody concentrations and incubation times to achieve the best results.
Precautions
Store at 2-8°C. Handle with care, and avoid contamination. Optimize antibody dilutions and incubation times for best results.
Validation Images