Goat Anti-Human Serum Albumin (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.76533-0.5mL | €212.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.76533-1mL | €1500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.76533-5mL | €1500.00 |
| Catalog No | CAB.76533 |
|---|---|
| Product Name | Goat Anti-Human Serum Albumin (Immune serum) |
| Isotype | N/A |
| Calculated MW | 66kDa |
| Immunogen | Human Serum Albumin |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Albumin is a globular unglycosylated serum protein of molecular weight 65，000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product， proalbumin， is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq， Jul 2008].This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat Anti-Human Serum Albumin (Immune serum) is a versatile antibody that can be applied in various immunoassays.
For ELISA, the recommended dilution is 1:5000-400000. Reagents or buffers required include coating buffer, blocking buffer, and substrate. Sample preparation involves coating the plate with antigen, followed by blocking and washing steps. Antibody incubation is performed by adding the diluted antibody to the wells, followed by washing and detection using a conjugated secondary antibody. Technical notes: Optimize the coating antigen concentration and antibody dilution to achieve the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents or buffers required include transfer buffer, blocking buffer, and washing buffer. Sample preparation involves separating the proteins by SDS-PAGE, followed by transferring the proteins to a membrane. Antibody incubation is performed by adding the diluted antibody to the membrane, followed by washing and detection using a conjugated secondary antibody. Technical notes: Use a suitable molecular weight marker to verify the target protein size.
For Double Immunodiffusion, the recommended dilution is 1:4-32. Reagents or buffers required include agar or agarose gel, and antigen. Sample preparation involves preparing the gel and adding the antigen and antibody to the designated wells. Technical notes: Optimize the concentration of the antigen and antibody to achieve the best precipitation line.
Precautions
Store at -20°C, handle with care, include positive and negative controls, and optimize antibody dilution for best results.
Validation Images