Anti-Cadherin 2 Monoclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.76757-50ul | €240.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.76757-100ul | €437.50 |
| Catalog No | CAB.76757 |
|---|---|
| Product Name | Anti-Cadherin 2 Monoclonal Antibody |
| Isotype | IgG2a |
| Calculated MW | N/A |
| Immunogen | Recombinant protein of human Cadherin 2 |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Reactivity | Human |
| Application | WB;IHC-P |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | PBS (pH 7.4) containing 50% glycerol，and 0.02% sodium azide. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
Background
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats，a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses，presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Protocol / Instructions
The Anti-Cadherin 2 Monoclonal Antibody is a high-quality antibody for detecting Cadherin 2 in various applications. Below are the protocols for the validated applications.
Reagents or buffers: RIPA buffer, SDS-PAGE gel, transfer buffer, blocking buffer, and ECL substrate.
Sample preparation: Prepare cell lysates using RIPA buffer and separate proteins by SDS-PAGE.
Antibody incubation: Incubate the membrane with the Anti-Cadherin 2 Monoclonal Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash the membrane with TBST and detect the signal using ECL substrate.
Technical notes: The expected band size is approximately 100 kDa.
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, blocking buffer, and DAB substrate.
Sample preparation: Prepare paraffin-embedded tissue sections and deparaffinize with xylene and ethanol.
Antibody incubation: Incubate the sections with the Anti-Cadherin 2 Monoclonal Antibody at a suitable dilution in blocking buffer for 1-2 hours at room temperature.
Washing or detection: Wash the sections with PBS and detect the signal using DAB substrate.
Technical notes: The antibody may require antigen retrieval using heat-mediated epitope retrieval.
Precautions
Store at 2-8°C. Handle with care, and avoid repeated freeze-thaw cycles. Optimize antibody dilution and incubation conditions for best results.
Validation Images