Goat Anti-Human IgG-Fc/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.76834-100ul | €45.00 |
| Catalog No | CAB.76834 |
|---|---|
| Product Name | Goat Anti-Human IgG-Fc/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgG-Fc |
| Application | IHC-P;IHC-F;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgG-Fc/HRP antibody is a versatile tool for detecting human IgG-Fc in various applications. Below are the protocols for each validated application.
Reagents or buffers: Transfer buffer, blocking buffer, wash buffer.
Sample preparation: Prepare protein samples and separate them by SDS-PAGE. Transfer the proteins to a membrane.
Antibody incubation: Incubate the membrane with the Goat Anti-Human IgG-Fc/HRP antibody at a dilution of 1:1000-10000 for 1-2 hours.
Washing or detection: Wash the membrane with wash buffer and detect using a chemiluminescent substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Fixative, antigen retrieval buffer, blocking buffer, chromogen.
Sample preparation: Fix and embed tissue samples, then section them.
Antibody incubation: Incubate the sections with the Goat Anti-Human IgG-Fc/HRP antibody at a dilution of 1:100-1000 for 30 minutes to 1 hour.
Washing or detection: Wash the sections with wash buffer and detect using a chromogen.
Technical notes: Use an appropriate antigen retrieval method for optimal staining.
Reagents or buffers: Fixative, permeabilization buffer, blocking buffer, fluorochrome.
Sample preparation: Fix and permeabilize cells or tissue samples.
Antibody incubation: Incubate the samples with the Goat Anti-Human IgG-Fc/HRP antibody at a dilution of 1:100-1000 for 30 minutes to 1 hour.
Washing or detection: Wash the samples with wash buffer and detect using a fluorochrome-conjugated secondary antibody.
Technical notes: Use an appropriate permeabilization method for optimal staining.
Reagents or buffers: Coating buffer, blocking buffer, wash buffer, substrate.
Sample preparation: Coat the plate with antigen and block non-specific binding sites.
Antibody incubation: Incubate the plate with the Goat Anti-Human IgG-Fc/HRP antibody at a dilution of 1:1000-10000 for 1-2 hours.
Washing or detection: Wash the plate with wash buffer and detect using a chromogenic substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for each application.
Validation Images