Anti-A2M Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.77023-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.77023-100ul | €335.00 |
| Catalog No | CAB.77023 |
|---|---|
| Product Name | Anti-A2M Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 163kDa |
| Immunogen | A synthetic peptide of human A2M |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | IHC-P |
| Subcellular Location | Secreted |
| Purification Method | CABfinity purification |
| Storage Buffer | pH7.4 PBS，0.05% NaN3，40% Glycerol. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits.
Protocol / Instructions
The Anti-A2M Polyclonal Antibody is a rabbit-derived antibody that recognizes human Alpha-2-macroglobulin (A2M).
Reagents or buffers: Xylene, ethanol, antigen retrieval buffer, and DAB substrate.
Sample preparation: Paraffin-embedded tissue sections are deparaffinized and rehydrated.
Antibody incubation: The primary antibody is applied at a dilution of 1:25-100 and incubated overnight at 4°C.
Washing or detection: The sections are washed and incubated with a secondary antibody conjugated to HRP, followed by DAB substrate.
Technical notes: Optimize the primary antibody dilution and incubation time for best results.
Precautions
Store at -20°C, handle with gloves, use positive and negative controls, and optimize antibody dilution for best results.
Validation Images