Goat anti-Bovine IgG (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.77553-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.77553-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.77553-5mL | €1125.00 |
| Catalog No | CAB.77553 |
|---|---|
| Product Name | Goat anti-Bovine IgG (Immune serum) |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | Bovine IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Bovine |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)， is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Bovine IgG (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:500-50000. Reagents needed include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the plate, followed by blocking and washing steps. The primary antibody is then added, and CABter incubation, the plate is washed, and the secondary antibody is added. Detection is achieved using a substrate, and the optical density is measured. Technical notes: it is essential to optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. Reagents needed include the sample, transfer buffer, and blocking buffer. Sample preparation involves separating the proteins using SDS-PAGE, followed by transfer onto a membrane. The membrane is then blocked, and the primary antibody is added. CABter incubation, the membrane is washed, and the secondary antibody is added. Detection is achieved using a chemiluminescent substrate. Technical notes: it is crucial to optimize the transfer conditions to ensure efficient transfer of the proteins.
For Double Immunodiffusion, the recommended dilution is 1:4-32. Reagents needed include the antigen of interest and agarose. Sample preparation involves preparing the antigen and antibody solutions, which are then added to the agarose gel. The gel is then incubated, allowing the antigen and antibody to diffuse and form a precipitin line. Technical notes: it is essential to optimize the concentration of the antigen and antibody to achieve a clear precipitin line.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize conditions for each application to minimize non-specific binding and ensure reliable results.
Validation Images