Donkey Anti-Goat IgG/Solar Fluor 750
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.77631-100ul | €87.50 |
| Catalog No | CAB.77631 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/Solar Fluor 750 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/Solar Fluor 750 is a secondary antibody used for detecting goat IgG primary antibodies.
Reagents or buffers: phosphate-buffered saline (PBS), secondary antibody, mounting medium, and a fluorescence microscope.
Sample preparation: fix and permeabilize cells or tissue sections, then block with a blocking buffer to reduce non-specific binding.
Antibody incubation: incubate samples with the primary antibody, followed by the Donkey Anti-Goat IgG/Solar Fluor 750 secondary antibody at a dilution of 1:50-500.
Washing or detection: wash samples with PBS to remove excess antibodies, then detect using a fluorescence microscope.
Technical notes: optimize the dilution of the secondary antibody and the incubation time to achieve the best signal-to-noise ratio.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/Solar Fluor 750 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:50-500.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time to minimize non-specific binding.
Validation Images