Rabbit anti-Biotin (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.77706-0.5mL | €550.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.77706-1mL | €3250.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.77706-5mL | €3250.00 |
| Catalog No | CAB.77706 |
|---|---|
| Product Name | Rabbit anti-Biotin (Immune serum) |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | Biotin |
| Public Immunogen Range | N/A |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Biotin |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Biotin is a water soluble vitamin， generally classified as a B complex vitamin， also called vitamin B4. CABter the initial discovery of biotin， nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria， yeasts， molds， algae， and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Rabbit anti-Biotin (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-100000. The reagents required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the plate, followed by blocking and washing steps. The antibody is then incubated with the antigen, and CABter washing, the substrate is added for detection. Technical notes: it is essential to optimize the coating and blocking conditions to minimize non-specific binding.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, transfer buffer, and blocking buffer. Sample preparation involves separating the proteins by SDS-PAGE, followed by transfer onto a membrane. The membrane is then blocked, and the antibody is incubated with the membrane. CABter washing, the membrane is incubated with a secondary antibody, and detection is performed using a substrate. Technical notes: the membrane should be handled carefully to prevent damage, and the blocking conditions should be optimized to minimize non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the antigen of interest and agarose. Sample preparation involves preparing the agarose gel and adding the antigen and antibody to the wells. The gel is then incubated, allowing the antigen and antibody to diffuse and form a precipitin line. Technical notes: the concentration of the antigen and antibody should be optimized to obtain a clear precipitin line, and the incubation time should be sufficient to allow for complete diffusion.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize conditions to minimize non-specific binding.
Validation Images