Mouse Anti Duck IgY Monoclonal antibody（Heavy Chain）/AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5ml | CAB.77917-0.5ml | €37.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5ml | CAB.77917-5ml | €300.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.77917-100ul | €87.50 |
| Catalog No | CAB.77917 |
|---|---|
| Product Name | Mouse Anti Duck IgY Monoclonal antibody（Heavy Chain）/AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Duck IgY（Heavy Chain） |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti Duck IgY Monoclonal antibody (Heavy Chain)/AP is a versatile tool for detecting Duck IgY (Heavy Chain) in various applications. Below are the protocols for each validated application.
Reagents or buffers: Tris-buffered saline with Tween 20 (TBST), skim milk, and AP substrate.
Sample preparation: Separate proteins by SDS-PAGE and transfer to a PVDF membrane.
Antibody incubation: Incubate the membrane with the Mouse Anti Duck IgY Monoclonal antibody (1:1000-10000) in TBST with 5% skim milk at 4°C overnight.
Washing or detection: Wash the membrane with TBST and detect using AP substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Carbonate buffer, blocking buffer, and AP substrate.
Sample preparation: Coat the ELISA plate with the antigen and block with blocking buffer.
Antibody incubation: Incubate the plate with the Mouse Anti Duck IgY Monoclonal antibody (1:1000-10000) in carbonate buffer at 37°C for 1 hour.
Washing or detection: Wash the plate with PBS and detect using AP substrate.
Technical notes: Use a standard curve to quantify the results.
Reagents or buffers: Xylene, ethanol, and AP substrate.
Sample preparation: Fix and embed tissue samples, then section and deparaffinize.
Antibody incubation: Incubate the sections with the Mouse Anti Duck IgY Monoclonal antibody (1:100-1000) at 4°C overnight.
Washing or detection: Wash the sections with PBS and detect using AP substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Reagents or buffers: Methanol, and AP substrate.
Sample preparation: Fix tissue samples, then permeabilize and block.
Antibody incubation: Incubate the samples with the Mouse Anti Duck IgY Monoclonal antibody (1:100-1000) at 4°C overnight.
Washing or detection: Wash the samples with PBS and detect using AP substrate.
Technical notes: Use a fluorescence quencher to reduce background.
Reagents or buffers: Methanol, and AP substrate.
Sample preparation: Fix and permeabilize cells, then block with blocking buffer.
Antibody incubation: Incubate the cells with the Mouse Anti Duck IgY Monoclonal antibody (1:100-1000) at 4°C overnight.
Washing or detection: Wash the cells with PBS and detect using AP substrate.
Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at -20°C, handle with care, and optimize antibody dilution for each application to avoid non-specific binding.
Validation Images