Anti-ALB Polyclonal Antibody
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.78389-50ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.78389-100ul | €335.00 |
| Catalog No | CAB.78389 |
|---|---|
| Product Name | Anti-ALB Polyclonal Antibody |
| Isotype | IgG |
| Calculated MW | 45/47/69kDa |
| Immunogen | Recombinant protein of human ALB |
| Public Immunogen Range | 25-609aa |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Reactivity | Rat;Human;Mouse |
| Application | ELISA;IF/ICC;WB |
| Subcellular Location | Secreted |
| Purification Method | CABfinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Background
This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor.
Protocol / Instructions
The Anti-ALB Polyclonal Antibody is a versatile tool for detecting ALB in various applications.
For ELISA, coat the plate with the antigen and block with 5% BSA in PBS. Incubate with the Anti-ALB Polyclonal Antibody at a dilution of 1:500-1000 in PBS with 1% BSA. Use a secondary antibody conjugated to HRP and detect with a chromogenic substrate.
For IF/ICC, fix cells with 4% PFA and permeabilize with 0.1% Triton X-100. Incubate with the Anti-ALB Polyclonal Antibody at a dilution of 1:50-200 in PBS with 1% BSA. Use a secondary antibody conjugated to a fluorescent dye and detect with a fluorescence microscope.
For WB, separate proteins by SDS-PAGE and transfer to a PVDF membrane. Block with 5% milk in TBS-T and incubate with the Anti-ALB Polyclonal Antibody at a dilution of 1:500-1000 in TBS-T with 1% milk. Use a secondary antibody conjugated to HRP and detect with a chemiluminescent substrate.
Technical notes: Optimize the antibody dilution and incubation time for each application to achieve the best results.
- Prepare lysates in SDS-compatible lysis buffer containing protease inhibitors, clarify by centrifugation, and denature samples at 95–100°C for 5 minutes.
- Resolve proteins by SDS-PAGE and transfer to PVDF or nitrocellulose membrane.
- Block with 5% BSA or nonfat milk in TBST for 1 hour at room temperature.
- Incubate the membrane with Anti-ALB Polyclonal Antibody diluted in antibody dilution buffer overnight at 4°C with gentle agitation. Recommended dilution: WB 1:500-1000. IF/ICC 1:50-200..
- Wash 3 times in TBST, then incubate with species-appropriate HRP-conjugated secondary antibody for 1 hour at room temperature.
- Wash again, develop with ECL substrate, and document target band intensity together with molecular weight marker and exposure conditions.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for each application.
Validation Images