Mouse Anti-Goat IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.78781-100ul | €200.00 |
| Catalog No | CAB.78781 |
|---|---|
| Product Name | Mouse Anti-Goat IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Goat IgG/APC is a versatile antibody that can be utilized in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
For IF, the recommended dilution is 1:100-1000. Reagents needed include the antibody, fluorescent mounting medium, and a fluorescence microscope. Sample preparation involves fixing and permeabilizing cells or tissue sections. The antibody is then incubated with the sample, followed by washing and detection using a fluorescence microscope. Technical notes: Optimize the antibody dilution for specific samples and ensure proper fixation and permeabilization for optimal staining.
For FCM, the recommended dilution is 1:100-1000. Required reagents include the antibody, flow cytometry buffer, and a flow cytometer. Sample preparation involves isolating cells and resuspending them in flow cytometry buffer. The antibody is then incubated with the cells, followed by washing and detection using a flow cytometer. Technical notes: Optimize the antibody dilution and incubation time for specific cell types and ensure proper compensation and gating for accurate analysis.
Use assay conditions validated for IF, apply Mouse Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation conditions for best results.
Validation Images