Goat anti-Equine IgG/Gold 20nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.79225-500ul | €42.50 |
| Catalog No | CAB.79225 |
|---|---|
| Product Name | Goat anti-Equine IgG/Gold 20nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | GICA;IGS;IEM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 20nm
Protocol / Instructions
The Goat anti-Equine IgG/Gold 20nm antibody is designed for use in various immunogold staining techniques, including Immunogold Silver Staining (IGS), Immunogold Cryosectioning (GICA), and Immunoelectron Microscopy (IEM). The recommended dilution ranges for these applications are provided, but optimal dilutions may vary depending on the specific experimental conditions.
Reagents or buffers: 2% paraformaldehyde, 0.1% glutaraldehyde in PBS, pH 7.4, and 2% uranyl acetate.
Sample preparation: Cryosectioning of fixed and embedded tissue samples.
Antibody incubation: Incubate sections with the Goat anti-Equine IgG/Gold 20nm antibody at a dilution of 1:20-200 in PBS for 1 hour at room temperature.
Washing or detection: Wash sections with PBS and then incubate with a silver enhancement solution to visualize the gold particles.
Technical notes: Optimize the silver enhancement time to achieve the desired level of staining.
Reagents or buffers: 2% paraformaldehyde, 0.1% glutaraldehyde in PBS, pH 7.4, and 2% uranyl acetate.
Sample preparation: Fixed and embedded tissue samples.
Antibody incubation: Incubate sections with the Goat anti-Equine IgG/Gold 20nm antibody at a dilution of 1:20-200 in PBS for 1 hour at room temperature.
Washing or detection: Wash sections with PBS and then incubate with a silver enhancement solution to visualize the gold particles.
Technical notes: Optimize the silver enhancement time to achieve the desired level of staining.
Reagents or buffers: 2% paraformaldehyde, 0.1% glutaraldehyde in PBS, pH 7.4, and 2% uranyl acetate.
Sample preparation: Fixed and embedded tissue samples.
Antibody incubation: Incubate sections with the Goat anti-Equine IgG/Gold 20nm antibody at a dilution of 1:20-200 in PBS for 1 hour at room temperature.
Washing or detection: Wash sections with PBS and then view with an electron microscope.
Technical notes: Optimize the antibody dilution and incubation time to achieve the desired level of labeling.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation times for best results.
Validation Images