Goat Anti-Human IgM/HRP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.1ml | CAB.79278-0.1ml | €42.50 |
| Catalog No | CAB.79278 |
|---|---|
| Product Name | Goat Anti-Human IgM/HRP |
| Isotype | N/A |
| Calculated MW | N/A |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human IgM |
| Application | IHC-F;ELISA;IHC-P;WB |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat Anti-Human IgM/HRP is a versatile antibody that can be used in various applications.
For Western Blotting, use the antibody at a dilution of 1:200-500. The reagents required include the antibody, blocking buffer, and a suitable substrate for HRP. Prepare the samples by lysing cells or tissues and separating proteins using SDS-PAGE. Transfer the proteins to a membrane and block with a blocking buffer. Incubate the membrane with the antibody, followed by washing and detection using a suitable substrate. Technical notes: Optimize the blocking buffer and washing conditions to minimize background.
For ELISA, use the antibody at a dilution of 1:500-1000. The reagents required include the antibody, coating buffer, blocking buffer, and a suitable substrate for HRP. Prepare the samples by coating the plates with the antigen and blocking with a blocking buffer. Incubate the plates with the antibody, followed by washing and detection using a suitable substrate. Technical notes: Optimize the coating and blocking conditions to minimize background.
For IHC-P, use the antibody at a dilution of 1:50. The reagents required include the antibody, antigen retrieval buffer, and a suitable chromogen. Prepare the samples by fixing and embedding tissues, followed by sectioning. Perform antigen retrieval and block with a blocking buffer. Incubate the sections with the antibody, followed by washing and detection using a suitable chromogen. Technical notes: Optimize the antigen retrieval and blocking conditions to minimize background.
For IHC-F, use the antibody at a dilution of 1:50. The reagents required include the antibody, blocking buffer, and a suitable fluorophore. Prepare the samples by fixing and permeabilizing cells or tissues. Block with a blocking buffer and incubate with the antibody, followed by washing and detection using a suitable fluorophore. Technical notes: Optimize the blocking and washing conditions to minimize background.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize conditions for each application to minimize background and ensure specificity.
Validation Images