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CATEGORIES

Goat anti-Chicken IgG/Gold 35nm

Product classification
Antibodies Colloidal Gold-Conjugated
Catalog No CAB.79351
Antibody overview
CAB.79351 Antibodies
Goat anti-Chicken IgG/Gold 35nm
Ordering
Available Variants
1 Variant
Change View
500ul
SKU
CAB.79351-500ul
Price
€42.50
Available – Dispatch in 14 days
Availability & Stock Pack SKU Price (EUR)
Available – Dispatch in 14 days
Global Network - International delivery
500ul CAB.79351-500ul €42.50
Quick page guide: Compare pack sizes, review validated applications and access product documents from this section.
Recommended Dilution
Dilution GICA=1:20-200
Validated Application Key
GICA
Validated application
GICA - Validated application
IGS
Validated application
IGS - Validated application
IEM
Validated application
IEM - Validated application
Technical Specifications
Catalog No CAB.79351
Product Name Goat anti-Chicken IgG/Gold 35nm
Isotype N/A
Calculated MW 150kDa
Immunogen N/A
Public Immunogen Range N/A
Host Goat
Clone Type N/A
Reactivity Chicken IgG
Application GICA;IGS;IEM
Subcellular Location N/A
Purification Method CABfinity purified by Protein G
Storage Buffer N/A
Storage Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles.

Background

The particle size is 35nm

Protocol / Instructions

Recommended workflow
Optimized handling and experimental guidance
Review the suggested sequence below before starting the assay to keep sample handling, incubation, and downstream interpretation aligned.
Lab-ready steps

The Goat anti-Chicken IgG/Gold 35nm antibody is designed for various immunogold labeling applications.

GICA Protocol
The Grid Immunogold Contrast Amplification (GICA) protocol involves labeling of antigens on grids for transmission electron microscopy.

Reagents or buffers: 1% BSA, 0.1% gelatin, and PBS.

Sample preparation: Prepare grids with the sample of interest.

Antibody incubation: Incubate the grids with the Goat anti-Chicken IgG/Gold 35nm antibody at a dilution of 1:20-200 for 1-2 hours at room temperature.

Washing or detection: Wash the grids with PBS and then detect using a transmission electron microscope.

Technical notes: Optimize the antibody dilution for the best results.

IGS Protocol
The Immunogold Silver Staining (IGS) protocol involves labeling of antigens on sections for light microscopy.

Reagents or buffers: 1% BSA, 0.1% gelatin, and PBS.

Sample preparation: Prepare sections with the sample of interest.

Antibody incubation: Incubate the sections with the Goat anti-Chicken IgG/Gold 35nm antibody at a dilution of 1:20-200 for 1-2 hours at room temperature.

Washing or detection: Wash the sections with PBS, followed by silver enhancement and then detect using a light microscope.

Technical notes: Optimize the antibody dilution and silver enhancement time for the best results.

IEM Protocol
The Immunoelectron Microscopy (IEM) protocol involves labeling of antigens on sections for electron microscopy.

Reagents or buffers: 1% BSA, 0.1% gelatin, and PBS.

Sample preparation: Prepare sections with the sample of interest.

Antibody incubation: Incubate the sections with the Goat anti-Chicken IgG/Gold 35nm antibody at a dilution of 1:20-200 for 1-2 hours at room temperature.

Washing or detection: Wash the sections with PBS and then detect using a transmission electron microscope.

Technical notes: Optimize the antibody dilution for the best results.

Precautions

Store at 4°C, handle with care, use appropriate controls, and optimize antibody dilution and detection methods for best results.

Validation Images

Goat anti-Chicken IgG/Gold 35nm Figure 1
Fig.1. Validation data.