Goat anti-Human Albumin /AP
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.79608-50ul | €210.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.79608-100ul | €100.00 |
| Catalog No | CAB.79608 |
|---|---|
| Product Name | Goat anti-Human Albumin /AP |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Human Albumin |
| Application | IHC-P;IHC-F;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Human Albumin /AP antibody is designed for various applications including Western Blotting, ELISA, Immunohistochemistry on paraffin sections (IHC-P), and Immunohistochemistry on frozen sections (IHC-F).
For Western Blotting, reconstitute the antibody according to the manufacturer's instructions. Prepare samples by lysing cells or tissues in an appropriate buffer, then separate proteins by SDS-PAGE. Transfer the proteins to a membrane and block with a blocking buffer. Incubate the membrane with the Goat anti-Human Albumin /AP antibody at a dilution of 1:1000-10000 in the blocking buffer. Wash the membrane with a washing buffer and detect using an alkaline phosphatase substrate. Technical notes: Optimize the blocking buffer and washing conditions to minimize background.
For IHC-P, dewax and rehydrate paraffin sections. Antigen retrieval may be necessary. Block the sections with a blocking buffer and incubate with the Goat anti-Human Albumin /AP antibody at a dilution of 1:100-1000. Wash the sections with a washing buffer and detect using an alkaline phosphatase substrate. Technical notes: Use a suitable chromogen to visualize the staining.
For IHC-F, fix frozen sections with an appropriate fixative. Block the sections with a blocking buffer and incubate with the Goat anti-Human Albumin /AP antibody at a dilution of 1:100-1000. Wash the sections with a washing buffer and detect using an alkaline phosphatase substrate. Technical notes: Optimize the fixation and permeabilization steps to preserve the antigenicity.
For ELISA, coat the plates with the antigen and block with a blocking buffer. Incubate the plates with the Goat anti-Human Albumin /AP antibody at a dilution of 1:1000-10000. Wash the plates with a washing buffer and detect using an alkaline phosphatase substrate. Technical notes: Optimize the coating and blocking conditions to minimize non-specific binding.
Precautions
Store at -20°C, handle with care, include positive and negative controls, and optimize antibody dilutions for each application to ensure specificity and sensitivity.
Validation Images