Donkey Anti-Guinea Pig IgG/Cy3
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20ul | CAB.80567-20ul | €100.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
50ul | CAB.80567-50ul | €500.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.80567-100ul | €200.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul(PBS Only) | CAB.80567-100ul(PBS Only) | €500.00 |
| Catalog No | CAB.80567 |
|---|---|
| Product Name | Donkey Anti-Guinea Pig IgG/Cy3 |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Guinea Pig IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Guinea Pig IgG/Cy3 is a secondary antibody used for detecting Guinea Pig IgG primary antibodies. It is conjugated to Cy3, a fluorescent dye that emits at 570 nm, making it suitable for various applications including Immunofluorescence (IF) and Flow Cytometry (FCM).
For IF, the following steps are recommended:
- Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde for fixation, 0.1% Triton X-100 for permeabilization, and a blocking buffer (e.g., 5% normal donkey serum in PBS).
- Sample preparation: Fix and permeabilize cells or tissue sections, then block with the blocking buffer.
- Antibody incubation: Incubate the sample with the primary Guinea Pig IgG antibody, followed by incubation with the Donkey Anti-Guinea Pig IgG/Cy3 at a dilution of 1:100 to 1:1000.
- Washing or detection: Wash the sample thoroughly with PBS, and then proceed with fluorescence microscopy.
- Technical notes: Optimize the dilution of the secondary antibody and the incubation time for the best signal-to-noise ratio.
For FCM, follow these steps:
- Reagents or buffers: PBS, a fixation buffer (e.g., 1% paraformaldehyde in PBS), and a permeabilization buffer if necessary.
- Sample preparation: Fix cells, and permeabilize if required for intracellular staining.
- Antibody incubation: Incubate cells with the primary Guinea Pig IgG antibody, followed by incubation with the Donkey Anti-Guinea Pig IgG/Cy3 at a dilution of 1:100 to 1:1000.
- Washing or detection: Wash cells with PBS, and then analyze by flow cytometry.
- Technical notes: Use appropriate controls to set gates and compensate for fluorescence spill-over.
Use assay conditions validated for IF, apply Donkey Anti-Guinea Pig IgG/Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Guinea Pig IgG/Cy3 at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IF=1:100-1000;
FCM=1:100-1000.
Precautions
Store at 4°C, handle with care, use appropriate controls, and optimize antibody dilutions for best results.
Validation Images