Goat anti-Human IgG (Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.80839-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.80839-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.80839-5mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
25T | CAB.80839-25T | €282.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.80839-100T | €677.50 |
| Catalog No | CAB.80839 |
|---|---|
| Product Name | Goat anti-Human IgG (Immune serum) |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | Human IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | Polyclonal Antibody |
| Reactivity | Human |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)， is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Goat anti-Human IgG (Immune serum) is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:4000-700000. The reagents required include the antigen of interest, coating buffer, blocking buffer, and substrate. Sample preparation involves coating the antigen onto the ELISA plate, followed by blocking and washing steps. The antibody is then incubated with the antigen, and CABter washing, the substrate is added for detection. Technical notes: it is essential to optimize the antibody dilution and incubation time for the best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the sample, transfer buffer, and blocking buffer. Sample preparation involves separating the proteins by SDS-PAGE, followed by transferring them onto a membrane. The membrane is then blocked, and the antibody is incubated with the membrane. CABter washing, the membrane is incubated with a secondary antibody, and the signal is detected using a substrate. Technical notes: the antibody dilution and incubation time may need to be optimized for the best results.
For Double Immunodiffusion, the recommended dilution is 1:8-64. The reagents required include the antigen of interest and agarose gel. Sample preparation involves preparing the antigen and antibody solutions, which are then placed in adjacent wells in the agarose gel. The gel is then incubated, allowing the antigen and antibody to diffuse and form a precipitin line. Technical notes: the concentration of the antigen and antibody may need to be optimized for the best results.
Precautions
Store at -20°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images