Donkey Anti-Goat IgG(Immune serum)
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
0.5mL | CAB.80932-0.5mL | €162.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mL | CAB.80932-1mL | €1125.00 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
5mL | CAB.80932-5mL | €1125.00 |
| Catalog No | CAB.80932 |
|---|---|
| Product Name | Donkey Anti-Goat IgG(Immune serum) |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | Goat IgG full length protein |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | Polyclonal Antibody |
| Reactivity | Goat |
| Application | ELISA;WB;Double Immunodiffusion |
| Subcellular Location | N/A |
| Purification Method | N/A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃，Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)， is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. This product is reddish transparent liquid， containing 0.03% Proclin300.
Protocol / Instructions
The Donkey Anti-Goat IgG is a versatile antibody that can be used in various applications, including ELISA, Western Blotting, and Double Immunodiffusion.
For ELISA, the recommended dilution is 1:5000-400000. The reagents required include the Donkey Anti-Goat IgG, Goat IgG antigen, and ELISA substrate. Sample preparation involves coating the ELISA plate with the Goat IgG antigen. The antibody incubation step involves adding the Donkey Anti-Goat IgG at the recommended dilution and incubating for 1-2 hours. Washing and detection involve washing the plate with PBS and adding the ELISA substrate. Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000. The reagents required include the Donkey Anti-Goat IgG, Goat IgG antigen, and Western Blotting substrate. Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane. The antibody incubation step involves adding the Donkey Anti-Goat IgG at the recommended dilution and incubating for 1-2 hours. Washing and detection involve washing the membrane with PBS and adding the Western Blotting substrate. Technical notes: Use a suitable blocking buffer to reduce non-specific binding.
For Double Immunodiffusion, the recommended dilution is 1:4-32. The reagents required include the Donkey Anti-Goat IgG and Goat IgG antigen. Sample preparation involves preparing the agar gel and adding the Goat IgG antigen and Donkey Anti-Goat IgG to separate wells. The antibody incubation step involves allowing the antibody and antigen to diffuse towards each other. Washing and detection involve observing the precipitation line. Technical notes: Optimize the antibody dilution and incubation time for best results.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for best results.
Validation Images