Donkey Anti-Goat IgG/APC
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
20T | CAB.81029-20T | €312.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100T | CAB.81029-100T | €782.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.81029-100ul | €200.00 |
| Catalog No | CAB.81029 |
|---|---|
| Product Name | Donkey Anti-Goat IgG/APC |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | N/A |
| Reactivity | Goat IgG |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Donkey Anti-Goat IgG/APC is a versatile antibody that can be used in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM).
For IF, the following steps are recommended:
Reagents or buffers: phosphate-buffered saline (PBS), 4% paraformaldehyde, and a mounting medium with DAPI.
Sample preparation: fix cells or tissue sections with 4% paraformaldehyde for 10-15 minutes, then permeabilize with 0.1% Triton X-100 for 10 minutes.
Antibody incubation: incubate samples with the Donkey Anti-Goat IgG/APC at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: wash samples three times with PBS, then detect using a fluorescence microscope.
Technical notes: optimize the antibody dilution and incubation time for specific experimental conditions.
For FCM, the following steps are recommended:
Reagents or buffers: PBS, 1% bovine serum albumin (BSA), and a fixative such as 1% paraformaldehyde.
Sample preparation: prepare cell suspensions in PBS, then block non-specific binding sites with 1% BSA for 10 minutes.
Antibody incubation: incubate cells with the Donkey Anti-Goat IgG/APC at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: wash cells twice with PBS, then analyze using a flow cytometer.
Technical notes: optimize the antibody dilution and incubation time for specific experimental conditions.
Use assay conditions validated for IF, apply Donkey Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Donkey Anti-Goat IgG/APC at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for specific experimental conditions to ensure accurate results.
Validation Images