Goat anti-Equine IgG/Bio
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.81500-100ul | €35.00 |
| Catalog No | CAB.81500 |
|---|---|
| Product Name | Goat anti-Equine IgG/Bio |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Equine IgG |
| Application | IF;IHC-F;IHC-P;ELISA;WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein G |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Goat anti-Equine IgG/Bio is a versatile antibody that can be used in various applications.
For Western blotting, use a dilution of 1:1000-10000. Prepare samples by lysing cells or tissues in a suitable buffer, then separate proteins by SDS-PAGE and transfer to a membrane. Incubate the membrane with the antibody in a blocking buffer for 1-2 hours, followed by washing and detection using a suitable conjugate.
For Immunohistochemistry on paraffin-embedded tissues, use a dilution of 1:100-1000. Deparaffinize and rehydrate tissue sections, then perform antigen retrieval if necessary. Incubate sections with the antibody in a humidified chamber for 30-60 minutes, followed by washing and detection using a suitable chromogen.
For Immunohistochemistry on frozen tissues, use a dilution of 1:100-1000. Fix and permeabilize tissue sections, then incubate with the antibody in a blocking buffer for 30-60 minutes. Follow with washing and detection using a suitable fluorophore.
For ELISA, use a dilution of 1:1000-10000. Coat plates with the antigen, then block and incubate with the antibody. Detect using a suitable conjugate and substrate.
For Immunofluorescence, use a dilution of 1:100-1000. Fix and permeabilize cells or tissues, then incubate with the antibody in a blocking buffer for 30-60 minutes. Follow with washing and detection using a suitable fluorophore.
Technical notes: Optimize antibody dilutions and incubation times for each specific application. Use appropriate controls to verify specificity and sensitivity.
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize conditions for each application.
Validation Images