Donkey Anti-Rabbit IgG
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
1mg | CAB.83451-1mg | €27.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
10mg | CAB.83451-10mg | €1562.50 |
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100mg | CAB.83451-100mg | €1562.50 |
| Catalog No | CAB.83451 |
|---|---|
| Product Name | Donkey Anti-Rabbit IgG |
| Isotype | IgG |
| Calculated MW | 150kDa |
| Immunogen | Rabbit full length protein |
| Public Immunogen Range | N/A |
| Host | Donkey |
| Clone Type | Polyclonal Antibody |
| Reactivity | Rabbit |
| Application | ELISA WB |
| Subcellular Location | N/A |
| Purification Method | CABfinity purification |
| Storage Buffer | Buffer: PBS with 0.03% Proclin300 （PH7.2-7.4). |
| Storage | Shipped at 4℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Immunoglobulin G (IgG)，is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions;to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Protocol / Instructions
The Donkey Anti-Rabbit IgG is a versatile antibody that can be used in various applications.
For ELISA, the recommended dilution is 1:5000-50000. The reagents required include the antigen of interest, ELISA plates, and a detection system.
Sample preparation involves coating the ELISA plates with the antigen and blocking non-specific binding sites.
The antibody incubation step involves adding the Donkey Anti-Rabbit IgG at the recommended dilution and incubating for 1-2 hours.
Washing and detection involve washing the plates with a washing buffer and adding a detection reagent.
Technical notes: Optimize the antibody dilution and incubation time for best results.
For Western Blotting, the recommended dilution is 1:1000-20000.
The reagents required include the protein sample, SDS-PAGE gels, and a transfer membrane.
Sample preparation involves separating the proteins by SDS-PAGE and transferring them to a membrane.
The antibody incubation step involves adding the Donkey Anti-Rabbit IgG at the recommended dilution and incubating for 1-2 hours.
Washing and detection involve washing the membrane with a washing buffer and adding a detection reagent.
Technical notes: Use a suitable blocking buffer to reduce non-specific binding and optimize the antibody dilution for best results.
Use assay conditions validated for ELISA WB, apply Donkey Anti-Rabbit IgG at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB 1:1000-20000.;ELISA:1:5000-50000..
Precautions
Store at 2-8°C. Handle with care, avoid repeated freeze-thaw cycles. Use appropriate controls and optimize antibody dilution for best results.
Validation Images