Mouse Anti-Rabbit IgM/PE
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
100ul | CAB.83555-100ul | €200.00 |
| Catalog No | CAB.83555 |
|---|---|
| Product Name | Mouse Anti-Rabbit IgM/PE |
| Isotype | N/A |
| Calculated MW | 900kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Mouse |
| Clone Type | N/A |
| Reactivity | Rabbit IgM |
| Application | IF;FCM |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Shipped at 2-8℃, Store at -20℃ at least one year (Avoid repeated freeze/thaw cycles). |
Background
Information not available.
Protocol / Instructions
The Mouse Anti-Rabbit IgM/PE antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are the protocols for each validated application.
Reagents or buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, and mounting medium.
Sample preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block non-specific binding sites.
Antibody incubation: Incubate samples with the Mouse Anti-Rabbit IgM/PE antibody at a dilution of 1:100-1000 in PBS for 1 hour at room temperature.
Washing or detection: Wash samples with PBS and mount with mounting medium. Visualize using a fluorescence microscope.
Technical notes: Optimize the antibody dilution and incubation time for specific applications.
Reagents or buffers: Phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA), and 0.1% sodium azide.
Sample preparation: Prepare single-cell suspensions and block non-specific binding sites with 1% BSA.
Antibody incubation: Incubate cells with the Mouse Anti-Rabbit IgM/PE antibody at a dilution of 1:100-1000 in PBS for 30 minutes on ice.
Washing or detection: Wash cells with PBS and analyze using a flow cytometer.
Technical notes: Optimize the antibody dilution and incubation time for specific cell types and instruments.
Use assay conditions validated for IF, apply Mouse Anti-Rabbit IgM/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Use assay conditions validated for FCM, apply Mouse Anti-Rabbit IgM/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;
IF=1:100-1000.
Precautions
Store at 2-8°C, handle with care, use appropriate controls, and optimize antibody dilution and incubation time for specific applications.
Validation Images