Goat Anti-Bovine IgG/Gold 20nm
Ordering
Available Variants| Availability & Stock | Pack | SKU | Price (EUR) |
|---|---|---|---|
|
Available – Dispatch in 14 days
Global Network - International delivery
|
500ul | CAB.83693-500ul | €42.50 |
| Catalog No | CAB.83693 |
|---|---|
| Product Name | Goat Anti-Bovine IgG/Gold 20nm |
| Isotype | N/A |
| Calculated MW | 150kDa |
| Immunogen | N/A |
| Public Immunogen Range | N/A |
| Host | Goat |
| Clone Type | N/A |
| Reactivity | Bovine IgG |
| Application | IEM;GICA;IGS |
| Subcellular Location | N/A |
| Purification Method | CABfinity purified by Protein A |
| Storage Buffer | N/A |
| Storage | Store at 2-8℃ for 3-6 months. Avoid repeated freeze/thaw cycles. |
Background
The particle size is 20nm
Protocol / Instructions
The Goat Anti-Bovine IgG/Gold 20nm antibody is designed for various applications including Immunoelectron Microscopy (IEM), Immunogold Silver Staining (IGS), and Grid Immunocytochemistry Assay (GICA).
Reagents or buffers: 1% glutaraldehyde, 0.1M phosphate buffer, pH 7.4, and 1% osmium tetroxide.
Sample preparation: Fix tissue samples with 1% glutaraldehyde, followed by postfixation with 1% osmium tetroxide.
Antibody incubation: Incubate samples with the Goat Anti-Bovine IgG/Gold 20nm antibody at a dilution of 1:20-200.
Washing or detection: Wash samples with 0.1M phosphate buffer, pH 7.4, and detect using a transmission electron microscope.
Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
Reagents or buffers: 2% glutaraldehyde, 0.1M phosphate buffer, pH 7.4, and silver enhancement reagents.
Sample preparation: Fix tissue samples with 2% glutaraldehyde.
Antibody incubation: Incubate samples with the Goat Anti-Bovine IgG/Gold 20nm antibody at a dilution of 1:20-200.
Washing or detection: Wash samples with 0.1M phosphate buffer, pH 7.4, and detect using silver enhancement reagents.
Technical notes: Optimize the silver enhancement time to achieve the best results.
Reagents or buffers: 1% glutaraldehyde, 0.1M phosphate buffer, pH 7.4, and 1% osmium tetroxide.
Sample preparation: Fix tissue samples with 1% glutaraldehyde, followed by postfixation with 1% osmium tetroxide.
Antibody incubation: Incubate samples with the Goat Anti-Bovine IgG/Gold 20nm antibody at a dilution of 1:20-200.
Washing or detection: Wash samples with 0.1M phosphate buffer, pH 7.4, and detect using a transmission electron microscope.
Technical notes: Optimize the antibody dilution and incubation time to achieve the best results.
Use assay conditions validated for IEM, apply Goat Anti-Bovine IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Use assay conditions validated for GICA, apply Goat Anti-Bovine IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Use assay conditions validated for IGS, apply Goat Anti-Bovine IgG/Gold 20nm at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: IEM=1:20-200;
IGS=1:20-200;
GICA=1:20-200.
Precautions
Store at 2-8°C, handle with care, and optimize antibody dilution and incubation time for best results.
Validation Images