Goat Anti-Guinea Pig IgG/PE

The Goat Anti-Guinea Pig IgG/PE antibody is designed for use in various applications, including Immunofluorescence (IF) and Flow Cytometry (FCM). Below are detailed protocols for each validated application.

Reagents or Buffers: Phosphate-buffered saline (PBS), 4% paraformaldehyde, 0.1% Triton X-100, blocking buffer (e.g., 5% normal goat serum), and mounting medium with DAPI.

Sample Preparation: Fix cells or tissue sections with 4% paraformaldehyde, permeabilize with 0.1% Triton X-100, and block with blocking buffer.

Antibody Incubation: Incubate samples with Goat Anti-Guinea Pig IgG/PE at a dilution of 1:100-1000 in blocking buffer for 1-2 hours at room temperature or overnight at 4°C.

Washing or Detection: Wash samples thoroughly with PBS, and then proceed with secondary detection if necessary. Mount samples with mounting medium containing DAPI for nuclear staining.

Technical Notes: Optimize the antibody dilution and incubation time based on the specific experimental conditions and the intensity of the fluorescence signal.

Reagents or Buffers: Phosphate-buffered saline (PBS), FCM buffer (e.g., PBS with 1% BSA and 0.1% sodium azide), and a flow cytometer.

Sample Preparation: Prepare cell suspensions in FCM buffer, and if necessary, fix and permeabilize cells according to the specific staining requirements.

Antibody Incubation: Incubate cells with Goat Anti-Guinea Pig IgG/PE at a dilution of 1:100-1000 in FCM buffer for 15-30 minutes at 4°C.

Washing or Detection: Wash cells with FCM buffer, and then analyze by flow cytometry.

Technical Notes: Use appropriate controls, such as isotype controls or unstained cells, to set gates and thresholds for analysis. Optimize the antibody dilution and incubation time to achieve the best signal-to-noise ratio.

Use assay conditions validated for IF, apply Goat Anti-Guinea Pig IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;

IF=1:100-1000.

Use assay conditions validated for FCM, apply Goat Anti-Guinea Pig IgG/PE at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: FCM=1:100-1000;

IF=1:100-1000.