Goat anti-Human Fibrinogen /Bio

The Goat anti-Human Fibrinogen/Bio antibody is a versatile tool for detecting Human Fibrinogen in various applications. Western Blotting Protocol For Western blotting, prepare samples by lysing cells or tissues in an appropriate buffer. Load 10-50 μg of protein onto an SDS-PAGE gel and transfer to a membrane. Block the membrane with 5% milk or BSA in TBS-T, then incubate with the Goat anti-Human Fibrinogen/Bio antibody at a dilution of 1:1000-10000 in blocking buffer for 1-2 hours. Wash the membrane with TBS-T and detect using a suitable secondary antibody conjugated to HRP or a fluorescent dye. ELISA Protocol For ELISA, coat plates with 1-10 μg/mL of antigen in coating buffer and incubate overnight. Block plates with 5% milk or BSA in PBS-T, then incubate with the Goat anti-Human Fibrinogen/Bio antibody at a dilution of 1:1000-10000 in blocking buffer for 1-2 hours. Wash plates with PBS-T and detect using a suitable secondary antibody conjugated to HRP or a fluorescent dye. Immunofluorescence (IF) Protocol For IF, fix cells or tissues with 4% PFA and permeabilize with 0.1-1% Triton X-100. Block samples with 5% milk or BSA in PBS, then incubate with the Goat anti-Human Fibrinogen/Bio antibody at a dilution of 1:100-1000 in blocking buffer for 1-2 hours. Wash samples with PBS and detect using a suitable secondary antibody conjugated to a fluorescent dye. Immunohistochemistry-Paraffin (IHC-P) Protocol For IHC-P, deparaffinize and rehydrate tissue sections, then perform antigen retrieval using a suitable method. Block sections with 5% milk or BSA in TBS, then incubate with the Goat anti-Human Fibrinogen/Bio antibody at a dilution of 1:100-1000 in blocking buffer for 1-2 hours. Wash sections with TBS and detect using a suitable secondary antibody conjugated to HRP or a fluorescent dye. Immunohistochemistry-Frozen (IHC-F) Protocol For IHC-F, fix tissue sections with 4% PFA and permeabilize with 0.1-1% Triton X-100. Block sections with 5% milk or BSA in PBS, then incubate with the Goat anti-Human Fibrinogen/Bio antibody at a dilution of 1:100-1000 in blocking buffer for 1-2 hours. Wash sections with PBS and detect using a suitable secondary antibody conjugated to HRP or a fluorescent dye. Technical notes: Optimize antibody dilutions and incubation times for each specific application. Use suitable controls, such as isotype controls or peptide competition, to verify specificity. **IF Protocol** Use assay conditions validated for IF, apply Goat anti-Human Fibrinogen /Bio at the recommended working dilution, include proper positive and negative controls, perform thorough wash steps, and document incubation time, temperature, and detection conditions for reproducible performance. Recommended dilution: WB=1:1000-10000; ELISA=1:1000-10000; IHC-P=1:100-1000; IHC-F=1:100-1000; IF=1:100-1000. **IHC-F Protocol** 1. Use fresh frozen sections fixed briefly with cold acetone or paraformaldehyde according to antigen sensitivity. 2. Bring slides to room temperature, wash in PBS, and block nonspecific binding before primary antibody incubation. 3. Apply Goat anti-Human Fibrinogen /Bio at the optimized dilution for 1 hour at room temperature or overnight at 4°C. Recommended dilution: WB=1:1000-10000; ELISA=1:1000-10000; IHC-P=1:100-1000; IHC-F=1:100-1000; IF=1:100-1000. 4. Wash in PBS or TBS, apply enzyme- or fluorophore-conjugated secondary antibody, and protect from light when fluorescence detection is.